Practical Guide: 1.3.1 Spray Treatment
Official Practical Guide – Swiss Bee Health Service (apiservice)
Practical Guide 1.3.1 describes Varroa control by spraying an oxalic acid solution (Oxuvar 5.7%). It explains the conditions relating to brood and outdoor temperature, the procedures for stacked-box and Swiss hives, and the necessary protective measures. Additional Swiss product information describes the conditions for spring and summer application in broodless colonies.
1. Purpose and principle
Oxalic acid spray treatment is used to control Varroa mites in colonies without brood or containing exclusively open brood. The treatment does not affect mites inside capped brood cells.
According to the Bee Health Service guide, the method is well tolerated by bees and achieves an effectiveness of approximately 95%.
The guide describes winter treatment, among other applications. However, spraying is not inherently restricted to winter. The specific requirements for summer use of Oxuvar are presented separately in section 4.
2. Conditions for application
Required equipment:
- Oxuvar 5.7% from Andermatt BioVet AG for spray treatment
- Acid-resistant rubber gloves and protective goggles
- FFP2 or FFP3 protective mask
- Long-sleeved clothing
- Sprayer
- Small measuring cup graduated in millilitres
- Mesh-protected Varroa floor insert for counting mites falling after winter treatment
- Bucket of water for promptly washing away any acid splashes
Treatment conditions according to the Bee Health Service:
- Treat only broodless colonies or colonies containing exclusively open brood.
- Winter treatment is carried out no earlier than November and no later than the end of December.
- Reliable verification of broodlessness requires opening the hives.
- If capped brood is present, remove it or postpone treatment until no capped brood remains.
- The outdoor temperature should be at least 8°C so that bees taking flight can return to the hive.
3. Procedure and recommendations
Prepare the Oxuvar 5.7% solution for spraying according to its package leaflet. Before treatment, use a measuring cup to determine how much solution is delivered with each spray trigger press.
Procedure for stacked-box hives:
- Put on protective gloves, goggles and mask.
- Prepare Oxuvar 5.7% according to the package leaflet.
- Spray each side of the frames with 3–4 ml of solution.
- Spray bees remaining on the hive walls.
- Close the hive floor if it is open.
- For winter treatment, insert a mesh-protected Varroa floor insert.
Procedure for Swiss hives:
- Put on protective gloves, goggles and mask.
- Prepare Oxuvar 5.7% according to the package leaflet.
- Hang all frames on the frame holder.
- Spray the bees remaining on the hive walls.
- When replacing the frames, spray each side with 3–4 ml of oxalic acid solution.
- Close the hive floor if it is open.
- For winter treatment, insert a mesh-protected Varroa floor insert.
When spraying frames containing open brood, use a 45° angle to avoid directly spraying the brood.
4. Precautions and limitations
Important safety instructions from the Bee Health Service
- Oxalic acid is highly irritating and harmful to human health.
- Wear protective goggles, acid-resistant rubber gloves, an appropriate protective mask and long-sleeved clothing.
- Use an FFP2 or FFP3 mask for spray treatment.
- Do not stand in the oxalic acid spray mist. Outdoors, a smoking bee smoker can help determine wind direction.
- Keep water available for promptly washing away any acid splashes.
- The treatment does not affect mites inside capped brood cells.
- According to the Bee Health Service, honey from colonies previously treated with oxalic acid during the same season should not be harvested or marketed because residues may be present.
Monitoring winter treatment: If more than 500 mites fall onto the Varroa floor insert within two weeks after oxalic acid winter treatment, the treatment must be repeated.
Additional Swiss product information – Summer use of Oxuvar 5.7%
The Swiss Oxuvar 5.7% package leaflet also provides for spray treatment in spring and summer (April to August) in broodless swarms, artificial swarms and other broodless colonies. Treatment is carried out when most bees are inside the hive (in the evening).
The package leaflet requires broodlessness for this application. The possibility mentioned in the Bee Health Service guide of treating colonies containing exclusively open brood must therefore not automatically be extended to all authorised summer applications.
Oxuvar must not be applied during a nectar flow. Combs used for honey production in the current year must not be sprayed. Following summer treatment of swarms, artificial swarms or broodless nucleus colonies, the package leaflet also specifies an autumn or winter treatment.
The package leaflet states that no withdrawal period is required for honey when the product is used correctly. This differs from the precautionary Bee Health Service recommendation not to harvest or market honey from colonies previously treated with oxalic acid in the same season. The current product information must be consulted.
Source: Swiss package leaflet for Oxuvar 5.7% ad us. vet. (Swissmedic 65'776), particularly sections 4, 5, 8, 10 and 12. This additional information comes from the veterinary product leaflet and does not constitute a standalone summer treatment protocol. Swiss veterinary product information for Oxuvar 5.7%.
5. Key takeaways
- Spray treatment is not restricted to winter. The Swiss Oxuvar product information also provides for spring and summer use in broodless colonies.
- The Bee Health Service guide describes treatment of broodless colonies or colonies containing exclusively open brood. For summer applications, the stricter conditions in the package leaflet must be followed.
- The outdoor temperature should be at least 8°C.
- Prepare Oxuvar 5.7% according to the package leaflet and spray each side of the frames with 3–4 ml of solution.
- Wear the required protective equipment and spray frames containing open brood at a 45° angle to avoid direct exposure of the brood.
- Repeat winter treatment if more than 500 mites fall within two weeks after treatment.
- For summer applications, observe the restrictions concerning nectar flow, honey production and the other requirements of the Swiss package leaflet.
6. Official source
Swiss Bee Health Service (apiservice)
Practical Guide 1.3.1 – Oxalic Acid Spray Treatment
Version: V 2507
Last ApiSavoir source review: 11 November 2026
Additional references mentioned in the official guide:
Oxuvar 5.7% package leaflet (German)
Oxuvar 5.7% spray treatment video (German)
Original Bee Health Service guide (PDF, German)
7. Further reading
- Practical Guide: 1.3.2 Oxalic Acid Trickling Treatment
- Practical Guide: 1.3.3 Oxalic Acid Vaporisation/Sublimation
- Practical Guide: 1.5.1 Measuring Natural Mite Fall
Application notice
This summary is provided for information purposes and does not replace the official Bee Health Service guide. Before practical application, consult the current product information, authorisation conditions and safety instructions.


